In order to study the effect of motion of multi-layer target on penetration of high-speed spherical projectile, the model of high-speed spherical projectile penetrating thin multi-layer target board was numerically simulated by the software ANSYS/LS-DYNA. Comparison between dynamic penetration of thin multilayer targets in different motion state and static penetration of one layer thin target with the same mass was made. When the target is stationary, the protective effect of the uniform thickness is better than that of the single-target. The protective effect is lower when moving in the horizontal negative direction than when in the forward direction and increases with increasing angle of incidence. When blasting plate moves normally in high speed, the defense on armor-piercing effect with different inclination shows a different rule.