In order to obtain the precise spatial position, takeoff eccentricity, taxiing speed and other parameters of the aircraft before takeoff, and provide an important evaluation basis for the performance of the aircraft landing gear, a precise spatial position measurement method of aircraft takeoff based on a single station camera is adopted. Taking the inertial navigation data extracted by the aircraft itself as the constraint condition, the forward intersection model is simplified, and the traditional intersection measurement of multiple cameras is changed into a single camera measurement, which is compared with the total station measurement data. The analysis results show that the measurement accuracy of the measurement method based on single station is better than 2 cm, which meets the requirements of flight test mission.