Aiming at the testing and evaluation problem of terminal guidance system of man-in-the loop of long-rang imaging guidance missile, a method using evaluation system which is based on high fidelity virtual prototype was brought forward. The evaluation system was established and the test was finished with the evaluation system. The test result shows that the descernment and the track capacity of the man-in-the-loop system can be evaluated by this evaluation system ecomomically and availably. This can offer evidence for the design of background project.