Missile simulation is very important for missile's experimental verification. A research was conducted based on a purpose of experimental verification. In this paper, a kind of six degree of freedom simulation for proportional navigation missile based on Matlab Simulink and Aerospace blockset was constructed, models of control system and aerodynamics were advanced. The key process and detailed methods were introduced, and formulae were given. Some pieces of advice were given for its application in verification. At last, the quantitative results and pictures of a kind of missile simulation were given through an example. The result of simulation shows the program and link are correct.