Systematical evaluation of anti-interference performance is an important task of test and evaluation of anti-ship missile. First, the analysis on process and simulation result was conducted, and corresponding evaluation specifications were established; then, the test program optimization was made by the application of uniform design in the background of anti-interference simulation of active suppression jamming. Finally, the research on anti-interference performance of anti-ship missile was conducted based on regression analysis. The test result shows that the thoughts and methods researched in this paper possess general applicability, and can provide particular technological accumulation and referential importance.