In order to test and evaluate jamming effect of electronic warfare weapons on missiles, a method that combines seeker captive flight jamming tests and missile flight simulation tests was proposed, in which seeker jamming effect model was constructed based on data of seeker captive flight jamming tests and immitted into missile digital simulation system to perform large number of missile flight simulation tests under electronic jamming. The method was demonstrated and validated by test and evaluation of jamming effect of a smokescreen device on TV guidance missiles. The results show that the method has the advantages of easy operation, low expense and high creditability.